FVIII-eGFP fusion proteins with preserved functional activity for the analysis of the early secretory pathway of FVIII.
Multimerin 1 inhibits thrombin generation and binds factor V and Va with high affinity.
Jeimy, S.B., Fuller, N., Quin-Allen, M.A., Tasneem, S. Segers, K., Stafford, A.R., Weitz, J.I., Camire, R.M., Nicolaes, G.A.F., Kane, W.H., Hayward, C.P.M. (2008) J Biol Chem, Thromb Haemost., 100(6):1058-67.PMID: 19132231
Screening Outside the Catalytic Site:
Inhibition of Macromolecular Inter-actions Through Structure-Based Virtual
Ligand Screening Experiments.
Sperandio O, Miteva MA, Segers K,
Nicolaes GA, Villoutreix BO. (2008) Open Biochem J. 2008;2:29-37, PMID: 18949072
Identification of surface epitopes of human coagulation factor Va which are important for interaction with activated protein C and heparin.
Segers, K., Dahlbäck, B., Rosing, J., Nicolaes, G.A.F. (2008) J Biol Chem, 283(33):22573-81 PMID: 18519572
Re-evaluation of the role of the protein S-C4b binding protein complex in activated protein C-catalyzed factor Va-inactivation.
Maurissen, L.F.A., Thomassen, M.C.L.G.D., Nicolaes, G.A.F., Dahlbäck, B., Tans, G., Rosing, J. and Hackeng, T.M. (2008) Blood 111(6):3034-41. PMID: 18160668
PROS1 Analysis in 87 Pedigrees with Hereditary Protein S Deficiency Demonstrates Striking Genotype-Phenotype Associations.
ten Kate, M., Platteel, M., Mulder, R., Terpstra, P. Nicolaes, G.A.F., Reitsma, P., van der Steege, G., van der Meer, J. (2008) Human Mutation, 29(7), 939-47. PMID: 18435454
Screening Outside the Catalytic Site: Inhibition of Macromolecular Interactions Through Structure-Based Virtual Ligand Screening Experiments.
Sperandio,O., Miteva, M.A., Segers, K., Nicolaes, G.A.F., Villoutreix, B.O. (2008) The Open Biochemistry Journal, 2, 29-37.
doi: 10.2174/1874091X00802010029
Defining the structure of membrane-bound human blood coagulation Factor Va.
Stoilova-McPhie, S., Awosile. F., Parmenter, C.D.J., Segers, K., Villoutreix, B.O., Nicolaes, G.A.F. (2008)
J Thromb Haemost. 6: 76-82. PMID: 17949476
2007
The role of thrombin exosites I and II in the activation of human coagulation factor V.
Segers, K., Dahlbäck, B., Bock, P.E., Tans, G.,Rosing, J., Nicolaes, G.A.F. (2007) J Biol Chem. 282(47):33915-33924. PMID: 17878169
Coagulation factor V and thrombophilia: background and mechanisms.
Segers, K., Dahlbäck, B., Nicolaes, G.A.F. (2007) Thrombosis and Haemostasis, 98(3): 530-542. PMID: 17849041
Design of protein-membrane interaction inhibitors by virtual ligand screening, proof of concept with the C2 domain of factor V.
Segers, K., Sperandio, O., Sack, M., Fischer, R., Miteva, M., Rosing, J., Nicolaes, G.A.F., Villoutreix, B.O.
(2007) Proc. Natl. Acad. Sci. 104 (31):12697-702. PMID: 17646652
Expression of allosteric linkage between the sodium ion binding site and exosite I of thrombin during prothrombin activation.
Kroh, H.K., Tans, G., Nicolaes, G.A.F., Rosing, J., Bock, P.E. (2007) JBC, J Biol Chem. 282(22):16095-104. PMID: 17430903
2006
Biochemical, molecular characterization and glyco-proteomic analyses of alpha1-proteinase inhibitor products used for replacement therapy.
Kolarich, D., Turecek, P.L., Weber, A., Mitterer, A., Graninger, M., Matthiessen, P., Nicolaes, G.A.F., Altmann, F., Schwarz, H.P. (2006) Transfusion 46(11):1959-77. PMID: 17076852
A structural model of the SHBG domain of human variant protein S Heerlen.
Nicolaes, G.A.F., Hackeng, T.M., Segers, K., Rosing, J. (2006) Thromb Haemost. 96(4):538-40. PMID: 17003935
Structural Models of the Snake Venom Factor V activators from Daboia russelli and Daboia lebetina.
Segers, K., Rosing, J., Nicolaes, G.A.F. (2006). Proteins 64(4):968-84. PMID: 16807918
Activation of Factor V by Venom Proteases.
Nicolaes,G.A.F., Rosing, J. (2006) Toxin Reviews 25(3):217-234. DOI: 10.1080/15569540600567289
2004
Identification of the MMRN1 binding region within the C2 domain of human factor V.
Jeimy, S.B., Woram, R.A., Fuller, N., Quinn-Allen, M.A., Nicolaes, G.A.F., Dahlbäck, B., Kane, W.H., Hayward, C.P. (2004) J Biol Chem., 279(49):51466-71. PMID: 15452129
Altered inactivation pathway of factor Va by activated protein C in the presence of heparin.
Nicolaes G.A.F., Sörensen, K.W., Friedrich, U., Tans, G., Rosing, J., Autin L. , Dahlbäck B., Villoutreix B.O. (2004) Eur J Biochem., 271(13):2724-36. PMID: 15206937
Impaired APC-cofactor activity of factor V is a major component of the APC resistance associated with the FV Leiden (R506Q) and R2 (H1299R) mutations. Castoldi, E., Brugge, J.M., Nicolaes, G.A.F., Tans, G., Rosing, J. (2004) Blood, 103(11):4173-9. PMID: 14976057
Functional Properties of Recombinant Factor V mutated in a Potential Calcium-Binding Site.
Sörensen, K.W., Nicolaes, G.A.F., Villoutreix, B.O., Yamazaki, T., Tans, G., Rosing, J., Dahlbäck, B. (2004) Biochemistry, 43(19):5803-10. PMID: 15134454
Welke factor laat ons bloed stollen?
Nicolaes, G.A.F. (2004) Chemisch2Magazine, 100(2): 18-19 (in Dutch)
Theoretical and experimental study of the Asp2194Gly mutation in the C2 domain of coagulation factor V.
Miteva, M.A., Brugge, J.M., Rosing, J., Nicolaes, G.A.F., Villoutreix, B.O. (2004) Biophys. J., 86(1): 488-98. PMID: 14695293
2003
Congenital and Acquired Activated Protein C Resistance
Nicolaes, G.A.F., Dahlbäck, B. (2003). Seminars in Vascular Medicine, 3, 33-46. PMID: 15199491
Activated protein C resistance (FVLeiden) and thrombosis: factor V mutations causing hypercoagulable states.
Nicolaes, G.A.F., Dahlbäck, B. (2003) Hematology and Oncology Clinics of North America, 17, 1-25. PMID: 12627662
2002
Structure-function studies of blood coagulation proteins using recombinant human factor V molecules
Nicolaes, G.A.F., Tans, G., Rosing, J. Progress Report CARIM, University Maastricht (2002) 50-54.
Molecular basis of quantitative factor V deficiency associated with factor V R2 haplotype
Govers-Riemslag, J., Castoldi, E., Nicolaes, G.A.F., Tans, G., Rosing, J. (2002) Thrombosis and Haemostasis, 88, 444-449.
Factor V and Thrombotic disease: description of a Janus-faced protein
Nicolaes, G.A.F., Dahlbäck, B. (2002) Arteriosclerosis, Thrombosis, and Vascular Biology, 22(4), 530-538. PMID: 11950687
2001
Factor V
Nicolaes G.A.F., Dahlbäck B. (2001) Encyclopedia of Molecular Medicine, T.E. Creighton, ed., John Wiley and Sons, Inc., New York, 1231-1234. ISBN 10: 0471374946
Introduction to structural bioinformatics; applications to blood coagulation factor V and factor VIII
Bruno O. Villoutreix, Gerry A. F. Nicolaes, Martine Aiach (2001) Hématologie, 7(4), 287-296. (in French) link
Secondary substrate-binding exosite in the serine protease
domain of activated protein C important for cleavage at Arg-506 but not at
Arg-306 in factor Va.
Friedrich U., Nicolaes G.A.F., Villoutreix B.O.,
Dahlbäck B. (2001) Journal of Biological Chemistry, 276(25), 23105-23108.
2000
The second Laminin G-type domain of protein S is indispensable for full cofactor activity in activated protein C-catalyzed inactivation of factor Va and factor VIIIa.
Evenäs, P., Garcia de Frutos, P., Nicolaes, G.A.F., Dahlbäck, B. (2000) Thrombosis and Haemostasis, 84(2),271-277. PMID: 10959700
Mutations in a potential phospholipid binding loop in the C2 domain of factor V affecting the assembly of the prothrombinase complex.
Nicolaes, G.A.F., Villoutreix, B.O., Dahlbäck, B. (2000) Blood Coagulation & Fibrinolysis, 11, 89-100. PMID: 10691103
1999
Acquired APC resistance and oral contraceptives: A randomised cross-over study of two low dose oral contraceptives.
Rosing, J., Middeldorp, S., Curvers, J., Thomassen, M.C.L.G.D., Nicolaes, G.A.F., Meijers, J., Bouma, B.N., Büller, H.R., Prins, M.H., Tans, G., (1999) the Lancet, 354, 2036-2040. PMID: 10636369
Partial glycosylation of Asn2181 in human factor V as cause of molecular and functional heterogeneity.
Nicolaes, G.A.F., Villoutreix, B.O., Dahlbäck, B. (1999) Biochemistry, 38(41), 13584-13591. PMID: 10521265
Acquired APC resistance and oral contraceptives: Differences between two functional tests.
Curvers, J., Thomassen, M.C.L.G.D., Nicolaes, G.A.F., van Oerle, R., Hamulyak, K., Hemker, H.C., Tans, G., Rosing, J. (1999) British Journal of Haematology, 105(1), 88-94. PMID: 10233368
1997
Regulation of thrombin formation by activated protein C: effect of the FVLeiden mutation.
Tans, G., Nicolaes, G.A.F., Rosing, J. (1997) Seminars in Hematology, 34(3), 244-255. PMID: 9241709
Human factor Va1 and Va2: Properties in the procoagulant- and anticoagulant pathways.
Hoekema, L, Nicolaes, G.A.F., Tans, G., Hemker, H.C., Rosing, J. (1997) Biochemistry, 36(11), 3331-3335. PMID: 9116011
Oral Contraceptives and venous thrombosis: different sensitivities to activated protein C in women using second and third generation oral contraceptives.
Rosing, J., Tans, G., Nicolaes, G.A.F., Thomassen M.C.L.G.D., Van Oerle, R., Van der Ploeg, P.M.E.N., Heijnen, P., Hamulyak, K., Hemker, H.C. (1997) British Journal of Haematology 97, 233-238. PMID: 9136971
Effect of activated protein C on thrombin generation and on the thrombin potential in the plasma of normal and APC resistant individuals
Nicolaes, G.A.F., Thomassen, M.C.L.G.D., Tans, G., Rosing, J., Hemker, H.C., (1997) Blood Coagulation & Fibrinolysis 8, 28-38.
1996
A prothrombinase-based assay for detection of resistance to activated protein C
Nicolaes, G.A.F., Thomassen, M.C.L.G.D., van Oerle, R., Hamulyak, K., Hemker, H.C., Tans, G., Rosing, J. (1996) Thrombosis and Haemostasis 76(3), 404-410. PMID: 8883278
1995
Effects of protein S and factor Xa on peptide bond cleavages during inactivation of factor Va and factor VaR506Q by activated protein C.
Rosing, J., Hoekema, L., Nicolaes, G.A.F., Thomassen, M.C.L.G.D., Hemker, H.C., Varadi, K., Schwarz, H.P., Tans, G. (1995) Journal of Biological Chemistry 270, 27852-27858. PMID: 7499257
Peptide bond cleavages and loss of functional activity during inactivation of factor Va and factor VaR506Q by activated protein C
Nicolaes, G.A.F., Tans, G., Thomassen, M.C.L.G.D., Hemker, H.C., Pabinger, I., Varadi, K., Schwarz, H.P., Rosing, J. (1995) Journal of Biological Chemistry 270, 21158-21166. PMID: 7673148
1994
Activation of human factor V by meizothrombin.
Tans,G., Nicolaes, G.A.F., Thomassen, M.C.L.G.D., Hemker, H.C., van Zonneveld, A-J., Pannekoek, H., Rosing, J. (1994) Journal of Biological Chemistry 269, 15969-15972. PMID: 8206891
Acylation of porcine pancreatic PLA2 influences penetration and substrate head- group binding, depending on the position of the acylated lysine in the enzyme molecule.
Lugtigheid, R.B., Nicolaes, G.A.F., Veldhuizen, E.J.A., Slotboom, A.J., Verheij, H.M., de Haas, G.H. (1993) European Journal of Biochemistry 216(2), 519-525. PMID: 8375390